Effects of dietary soy isoflavone and soy saponin on growth performance, intestinal structure, intestinal immunity and gut microbiota community on rice field eel (Monopterus albus)

An eight-week feeding trial was conducted to evaluate the effects of soy isoflavone and saponin on growth performance, intestinal structure, immunity gut microbiota community rice field eel Monopterus albus (initial weight 20.12 ± 0.15 g). Six isonitrogenous isolipidic experimental feeds were formulated, including a positive control (55% fish meal, FM), negative (28.6% 37.2% soybean SBM) basic diet group 22.6% protein concentrate, SPC), rest supplemented with (40%, 2.5 g / kg, SI), (?98%, 2 SS) their mixed in (SI + SS). Compared FM, immune indices (acid phosphatase, alkaline complement 3, 4 immunoglobulin M), villus height muscular thickness mRNA (occludin-like, claudin 12, tight junction ZO-1-like, ZO-2-like, interleukin-10, transforming factor beta 1, 2) expression intestine M. fed SBM significantly decreased ( P < 0.05). Growth performance SPC better than that SBM. SPC, dietary (SI, SS, SI , serum phosphatase increased 0.05), M SS remarkably declined M, amounts goblet cells per root, shannon Based current results, there has trend damaged structure changed bacteria destroyed barrier, eventually induced inflammatory, feed more serious they single supplemented. • Dietary isoflavone, . Soy inflammatory. effect